Banana dna extraction lab report answers. banana lab report 2022-10-25
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Bananas are a popular fruit that is widely consumed all around the world. They are rich in nutrients and are an excellent source of dietary fiber, vitamin C, and potassium. In addition to their nutritional value, bananas are also of scientific interest because they contain DNA, the genetic material that carries the instructions for all the characteristics of an organism. Extracting DNA from a banana is a fun and educational laboratory activity that allows students to learn about the structure and function of DNA and how it can be extracted from a biological sample.
To extract DNA from a banana, you will need the following materials:
1 ripe banana
1 small plastic bag
1 blender or food processor
1 cup of water
1 tablespoon of salt
1/4 cup of rubbing alcohol
1 tablespoon of liquid soap or detergent
1 spoon or spatula
1 small funnel or strainer
1 glass jar or beaker
To begin the DNA extraction process, start by peeling the banana and placing the peeled fruit into a small plastic bag. Next, add the cup of water and the tablespoon of salt to the bag and seal it tightly. Use your hands to mash the contents of the bag until the banana is fully mashed and the mixture has a smooth, homogenous consistency.
Next, pour the mashed banana mixture into a blender or food processor and blend it until it is fully liquified. Pour the resulting liquid into a glass jar or beaker.
Add one tablespoon of liquid soap or detergent to the jar or beaker, and stir the mixture gently but thoroughly. The soap or detergent helps to break down the cell walls of the banana, which releases the DNA from the cells.
Now, add 1/4 cup of rubbing alcohol to the jar or beaker. The alcohol will help to separate the DNA from the other cell components. As you add the alcohol, you should see a white, cloudy layer forming at the top of the mixture. This layer is the DNA.
To collect the DNA, use a spoon or spatula to gently scoop the cloudy layer off the top of the mixture and place it into a small funnel or strainer. The DNA will be collected in the funnel or strainer, while the other cell components will pass through.
Once you have collected the DNA, you can observe it under a microscope or perform additional experiments to further study its properties and functions.
In conclusion, extracting DNA from a banana is a simple and fun laboratory activity that allows students to learn about the structure and function of DNA and how it can be extracted from a biological sample. By following the steps outlined above, you can easily extract DNA from a banana and use it for a variety of scientific investigations.
Biology DNA Lab lab answers
The proteins are made up of amino acids, DNA… Pglo Transformation Lab Report DNA, shorthanded for Deoxyribonucleic acid is a nucleic acid contains instructions for the development, functionality, and maintenance of new cells. Hypothesis: If the DNA from an equal amount of strawberry ,banana and an onion were extracted, then the banana sample would yield the most DNA and the onion sample would yield the least amount of DNA. Lastly we can use a toothpick or popsicle stick to gently remove the DNA out of the solution. Each strand of the helix consists of a linear polymer made up of genetic building blocks called nucleotides. Dish soap "cuts through grease" because it actually separates those greasy molecules from each other.
Activity 1 - DNA Extraction. Then the bananas were mushed more. This order determines various genetic information, but it cannot modify the general structure of the DNA on a vast scale, as seen by the white precipitate formed in this lab. After several minutes, the DNA should be suspended in the alcohol layer. They produce molecules called proteins that do most of the work in the body.
bio181 banana dna extraction childhealthpolicy.vumc.org
Step 4: The mixture was transferred to a beaker, put into the hot water bath, and left in it for approximately 12mins. Banana Dna Extraction Ask A Biologist In this activity students extract DNA and also some RNA from bananas. While you may know of the double-helix structure of DNA, you can't see that structure with the naked eye. Its natural shape is called a double helix and when seen under extremely high-powered microscopes, it looks kind of like a ladder twisted into a spiral shape. The enzymes are proteins and the particular stretch of DNA forms the code needed for production of the enzymes and other proteins.
A solid substance separated from a solution by physical or chemical change. It is the master code or blueprint for the organism. Understand the importance of DNA Worksheet or Lab Report. The solution had to be blended for 15 to 20 seconds, until it became a dense mixture. Introduction: The process of isolating DNA from a cell is the first step of many laboratory procedures in biotechnology. Although nowadays very few errors are made due to scientists using biotechnology equipment that allows them to look at DNA to see exactly how organisms are different, and find out how they work.
Each strand is made up of a chain of nucleotides. Add the cut banana fruit to the bag remove the air, seal and then start squishing it up. After waiting for the DNA to settle, you are able to analyze the DNA. Have the students read the introduction section of t he Strawberry DNA Extraction worksheet. Cited: Simon, Eric J. HYPOTHESIS: If the DNA in the banana is coiled around proteins in the nucleus then using dish soap, salt, and alcohol will release because the mix of these materials will lyse the nuclear membrane causing the release of DNA to precipitate into the alcohol. If you could take all the DNA out of your body would it fit on a plate.
What does soap do when you wash your hands. This information can be used to improve crops so that they are more resistant to disease insect invasion or changes in climate. Do not mix but allow the solutions to layer. DNA or deoxyribonucleic acid is found in all living things. If you took all of the DNA out of some middle-sized organism or part of an organism, like a piece of fruit ,you could see and even touch DNA. The water was left until it reached between 55 °C and 60 °C.
Banana DNA Extraction Lab childhealthpolicy.vumc.org
During cell division this code is copied and passed to new cells. The order in which the procedure is stated is very important because the cells must first be broken down in order to release the DNA to be seen. Accessed 4 June 2021. Add 1-½ tablespoons 8mL of ice-cold 70-95% isopropyl alcohol by pouring it gently down the side of the tilted container. Accessed 4 June 2021. We are able to observe the extraction of genomic DNA from plant cells.
You are trying to create a layer of alcohol that floats on the top of the water solution. Assume that a base substitution occurred in the 7th base position counting from the 5' end of that coding region. The salt enables the DNA strands to come together or aggregate. Proteinase K can aid in the hydrolysis decomposition of proteins and histones by interacting with water of proteins and histones, allowing pure DNA to be left alone. The dish soap is amphiphilic, meaning it has a hydrophilic head and a hydrophobic tail.
This experiment can help them in their future careers, as they will be able to apply this knowledge to real-life situations. Be sure to only remove the DNA from the top layer. As part of the chromosomes, the information contained in genes can be transmitted faithfully by parents through gametes to their offspring. DNA can be extracted and observed. For example, a strawberry is an octoploid contains 8 sets of chromosomes and a banana is a triploid contains 3 sets of chromosomes , therefore one would expect more DNA to be extracted from a strawberry than a banana 16. Stir until the salt is completely dissolved.
The two strands are held together by hydrogen bonds between adenine and thymine and between guanine and cytosine. How do we take out the DNA1. While the banana solution was being mixed, a number two cone coffee filter was laced inside a second five-ounce cup. The four nitrogenous are A adenine , G guanine , T thymine and C cytosine. How do we take out the DNA1.