Hydrogen peroxide is a chemical compound that is often used as a disinfectant due to its ability to kill bacteria and viruses. However, hydrogen peroxide can also be harmful to cells, as it can cause oxidative damage to proteins and DNA. To protect cells from the harmful effects of hydrogen peroxide, many organisms, including humans, produce an enzyme called catalase.
Catalase is an enzyme that catalyzes the breakdown of hydrogen peroxide into water and oxygen. This reaction is important because it helps to prevent hydrogen peroxide from accumulating in cells and causing damage. The activity of catalase is dependent on the concentration of hydrogen peroxide, with higher concentrations of hydrogen peroxide leading to increased enzyme activity.
To study the effect of hydrogen peroxide concentration on catalase activity, a simple experiment can be performed using potato slices as the source of catalase. To begin, potato slices are cut and placed into test tubes containing different concentrations of hydrogen peroxide. The test tubes are then sealed and placed in a water bath at 37 degrees Celsius to mimic the temperature of the human body.
After a set amount of time, the amount of oxygen produced by the reaction can be measured using a gas pressure sensor or by collecting the gas in a graduated cylinder. The results can then be plotted on a graph to show the relationship between hydrogen peroxide concentration and catalase activity.
One potential outcome of this experiment is that as the concentration of hydrogen peroxide increases, the activity of catalase also increases. This relationship may be described by a linear or exponential curve, depending on the specific enzymes and substrates being studied.
In addition to studying the effect of hydrogen peroxide concentration on catalase activity, this experiment can also be used to investigate other variables that may affect enzyme activity, such as temperature and pH. By altering these variables and repeating the experiment, researchers can gain a better understanding of the factors that influence enzyme activity and how enzymes can be used to catalyze reactions in different biological and industrial settings.
In conclusion, the concentration of hydrogen peroxide can have a significant impact on the activity of catalase, an enzyme that plays a vital role in protecting cells from oxidative damage. By conducting experiments to study the relationship between hydrogen peroxide concentration and catalase activity, researchers can gain insights into the mechanisms of enzyme action and how enzymes can be used to catalyze reactions in a variety of settings.
Catalase Lab Report
RESULTS Below are the results for the reaction of hydrogen peroxide in the presence of potato catalase. Catalase is an enzyme present in the human liver and functions at Enzyme Catalase Reaction Lab use enzymes in order to break down potentially harmful materials in the body and to carry out chemical reactions. I use 35% for only a few things: I use it in baths, I use it in my garden sprayer, and I use it to make 3%. Each enzyme catalyses only one type of reaction. The controlled variable in this investigation were the temperature of the room, size and number of potato discs used for the experiment because this was the variable that made the results of the investigation valid. Cells need them to survive and to function.
Effect of Ph Level and Hydrogen Peroxide on Catalase
Rate of reaction is measured by Average Once the graphs have been drawn trends will be looked for and identified. Evaluation: There are no errors present in this experiment that have the potential to completely skew the data and results, as all results matched the denaturation of the enzyme. The gas syringe only had measurements for 1 whole number this could have affected the results because no decimal places could be used. Increasing the concentration of the reactants will increase the frequency of collisions between the two reactants. Water treatment applications are often measured in parts per million -- which is even more dilute.
Where you should go to buy peroxide may also depend on what grade you are looking for. This means that decomposition of hydrogen peroxide happened. When you add water, you change the concentration. It will also allow for a third replicate to be taken because the time allocated for the investigation is brief and without the reaction being speeded up there may only be time for one or two experiments for each concentration. Moreover, enzymes are specific in action.
As the temperature varied from catalases optimal of 37° C, the reaction rate of catalase decreased. In this experiment, the hypothesis was also addressed. An electrically monitored water bath would be useful this could keep the water in the correct temperature range accurately. The rate of reaction between Catalase and the hydrogen peroxide H2O2 can vary depending on many factors including the enzyme concentration in a solution. Some applications use 3%, such as cleaning floors, windows, and countertops.
Effect of concentration of H2O2 on the enzyme catalase Essay Example
Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Add this to the conical flask. The experiment was repeated 3 times to and an average was taken improve accuracy. For home use 35% is the strongest concentration that I'm aware of that is sold, used or stored. All home applications of 35% hydrogen peroxide require a great amount of dilution.
Effect of concentration of hydrogen peroxide on catalase childhealthpolicy.vumc.org
The line of best fit shows that, after this point the rate of the reaction decreased. The hypothesis was tested by adding 50ml of hydrogen peroxide H2O2 into four different concentrations of Catalase 25%, 50%, 75%, and 100%. Due to enzymes are proteins. Enzymes have an optimum pH, and the further the pH level is from this optimum, the more disturbed the active site will become, decreasing enzyme activity Figure 4 Royal Society of Chemistry, 2004. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. It will be heavier and larger to carry. The solution will be measured in a measuring cylinder.
How does catalase concentration affect the rate of the reaction with hydrogen peroxide?
Conclusion: The effects that hydrogen peroxide and acetic acid have on the enzyme catalase were experimented with in this lab. As shown in the images from the results, there was some minor foam formation from the oxygen, but in comparison to the first glass, the enzyme reaction was nowhere near as strong as the first glass. What is the purpose of the catalase activity experiment? Practice setting the stir rate, making sure it remains fixed for the duration of each run. By measuring the distance of the water move in the pipette and the time of the experiment, we can find out the reaction rate. What is the effect of catalase on the decomposition of hydrogen peroxide? However, the effervescence and bubbles produced in test tube containing fresh liver is more as compared to test tube containing fresh potato. The Michaelis constant was calculated also from the mutual effects expected to occur between inhibitor and substrate and gave a mean value that was in good accord with the one above mentioned. We then continued to collect data on a laptop.
The slope of the tangent line is your initial rate of your reaction and should be recorded into your laboratory notebooks. Enzymes are catalysts that help to speed up the rate of reactions that otherwise would take longer periods of time to occur. What happens when you add hydrogen peroxide to an enzyme? The Gas Pressure sensor is connected to a Vernier computer interface Go-Link connector that plugs directly into any computer. Catalase acts as the catalyzing enzyme in the decomposition of hydrogen peroxide. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? A larger experimental group and controlled group could be experimented with if using liver, as liver is quite large, more results could be obtained.
This brings in a fairly large percentage error. During the experiment, the H2O2 rate will be changed and pressure will be measured using a gas pressure sensor. Practice with water several times to make sure you are comfortable injecting the enzyme into the reaction tube. At 30%, there were many active sites but insufficient number of substrate molecules to occupy the active site. Concentrations of hydrogen peroxide are most often stated as a percentage. In order to determine the value for the rate constant k the units for the rate and the units for the concentration of hydrogen peroxide have to have some consistency. If your pressure curve is not rising during the run you may have a leak and you should call this to the attention of your TA and figure out where the leak may be coming from.