Vitamin c determination by iodine titration lab report. Vitamin C Determination By Iodine [k546v281gwn8] 2022-10-10

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Vitamin C, also known as ascorbic acid, is a water-soluble vitamin that is essential for the proper functioning of the human body. It plays a key role in the synthesis of collagen, the main structural protein in the body, as well as in the synthesis of neurotransmitters and hormones. Vitamin C is also a powerful antioxidant, meaning it helps to protect cells from damage caused by free radicals.

The determination of vitamin C content in a sample can be done through a variety of methods, including colorimetry and spectrophotometry. However, one of the most commonly used methods is iodine titration. This method is based on the reaction between vitamin C and iodine, which results in the formation of a blue-black complex known as iodopurpurin.

In the iodine titration lab, a known volume of the sample containing vitamin C is placed in a flask. An iodine solution is then added to the flask, and the volume of the iodine solution required to react with all of the vitamin C in the sample is determined through titration. The endpoint of the titration is the point at which the iodine solution has completely reacted with the vitamin C in the sample, resulting in the formation of the iodopurpurin complex.

To determine the vitamin C content of the sample, a standard curve is prepared by titrating known concentrations of vitamin C with the iodine solution. The concentration of vitamin C in the sample can then be determined by comparing the volume of iodine solution required to react with the sample to the standard curve.

There are several advantages to using the iodine titration method for the determination of vitamin C. One of the main advantages is that it is a relatively simple and quick method that does not require specialized equipment. Additionally, the method is highly accurate and reproducible, making it a reliable method for the determination of vitamin C.

There are, however, some limitations to the iodine titration method. One limitation is that the method is not suitable for the determination of vitamin C in the presence of other reducing agents, as these agents can interfere with the reaction between vitamin C and iodine. Additionally, the method is not sensitive to low concentrations of vitamin C, making it less suitable for the analysis of samples with low vitamin C content.

Overall, the iodine titration method is a reliable and widely used method for the determination of vitamin C in a variety of samples. While it does have some limitations, it remains an important tool for the analysis of vitamin C content in a range of different samples.

Vitamin C Determination by Iodine Titration

vitamin c determination by iodine titration lab report

The results of the titration of iodine solution using ascorbic acid as a calibration standard determined the amount of Ascorbic Acid to be -2. If the results from your three runs are not within 5% of each other, you should repeat the standardization until you have three runs that are. This approach leads to a more reliable result than just using the values of one single titration since the impact on different errors on the measurements should be minimized. This is the case because we can obtain the greatest volume of juice by juicing an orange and also because again the ascorbic acid concentration is greatest in oranges. A few of the side impacts are the Macromolecules Lab Report 1486 Words 6 Pages The iodine test determines the presence of starch in biological materials. In general, projects that seek to only measure the amount of vitamin C in two or more samples for example, comparing apple and orange juice will not be graded as highly because they have a weak hypothesis.

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Lab 4

vitamin c determination by iodine titration lab report

MATERIALS · Starch indicator: Make a paste of 5 g of soluble starch and 5 mg of Hg 2 I 2 used to preserve the solution in 50 mL of distilled water. The amla organic product is accounted for to contain about 20 fold the amount of vitamin C as squeezed orange. Include in your proposal a list of the samples that you will analyze you will supply the samples and outline any sample preparation that is not described in the experimental section of this exercise. Average Concentration of Sodium Thiosulfate: 1 M TRIAL VOLUME OF SODIUM THIOSULF ATE mL ADDED MOLES OF SODIUM THIOSULF ATE MOLES OF IODINE IN SOLUTION MOLES OF IODINE TITRATED MOLES OF RESIDUAL IODINE MASS OF ASCORBIC ACID - VIT. The standardization of the iodine solution using ascorbic acid was accurate and precise however there was a calculation error that is exhibited in this report. The results of the titration of iodine solution using ascorbic acid as a calibration standard determined the amount of Ascorbic Acid to be -2. Standardization of the iodine solution with the vitamin C standard solution and sample solution.

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Determination of Vitamin C

vitamin c determination by iodine titration lab report

Besides plays a paramount role as an antioxidant and free radical scavenger, it has been suggested to be an effective antiviral agent 2. The blue-black color is the endpoint of the titration. Each pair will separately standardize the DCIP. Take the measurements you obtained and average them. Vitamin C Determination by Iodine Titration Vitamin C - Ascorbic Acid wikipedia Vitamin C ascorbic acid is an antioxidant that is essential for human nutrition. The concentration of the ascorbic acid in the fruit samples could be determined by referring to values of a standardized iodine solution in our calculations. Next, we obtained an Erlenmeyer flask for the titration.

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Determination of Vitamin C using Iodometric titration

vitamin c determination by iodine titration lab report

Pour the paste into 500 mL of boiling distilled water and boil until it is clear. Vitamin C is important because it is an essential antioxidant necessary for human nutrition. Compared to that limes and lemons are rather hard to eat and we would have to process them otherwise to obtain their vitamin c. The redox titration endpoint is determined by the first iodine excess that is complexed with starch, giving a deep blue-violet color. First, you will need to have an estimate of how much titrant DCIP will be used in your titration. There are several other things to consider as you design your experiment.

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Lab Report Vit C Titration new

vitamin c determination by iodine titration lab report

Therefore, you may need to perform some sample preparations, such as cooking, before coming to the laboratory. Potassium iodate is used as a titrant and is added to an ascorbic acid solution that contains strong acid and potassium iodide KI. However, since it is very hard to determine a the exact volume we disregarded this fact in our calculations and discussions and just assumed that the thickness of the peel would not alter our final result too much. Based on this value, the volume of the fruit juice samples, the volume of the whole fruits and the concentration of ascorbic acid in our fruit juice samples I calculated the number of fruits we would have to take to cover the 75g need, as well as the overall weight and volume the fruits we would have to take would take. Transfer the solution to a 600 ml beaker. Add distilled water to make a final solution of 100 ml in a volumetric flask.


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Vitamin C Determination by Iodine childhealthpolicy.vumc.org

vitamin c determination by iodine titration lab report

Include any modifications, especially ones prompted by your experience in the laboratory. That means if the concentration of vitamin c in the solution titrated with iodine solution the concentration of iodine we calculated might have been inaccurate. Then leaving Triiodide to oxidize Ascorbic Acid to form. Another human made mistake was that when we prepared the ascorbic acid solution for the standardization of the iodine solution we filled the Erlenmeyer flask up with a bit too much water. Iodine is relatively insoluble but by complexing Iodine with Iodide I 2 , Triiodide I 3 will result. Standardization of iodine solution was done with known amount of …show more content… Vitamin C titration is needed to perform this task in addition to standardizing of Iodine solution.

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Lab 4

vitamin c determination by iodine titration lab report

Fruits, vegetables, and organ meats e. Then 2 mL of starchindicator is added to complete the titration. Wash the filter with a few milliliters of distilled water. On the other hand, vitamin C is oxidized to dehydroascorbic acid reagents ratio is1:1 : The rest of the iodine is titrated with sodium thiosulfate. Add 10 drops of 1% starch solution. Record the initial volume.

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Vitamin C Titration Lab Report

vitamin c determination by iodine titration lab report

Determine how much titrant was required for your standard. The concentration of the acid we used in class will be sampled with a standardize solution such as sodium hydroxide with an environmentally indicator to show the physical change of color that occurs to the solution by the acid. Many fruits and vegetables contain vitamin C, but cooking destroys the vitamin, so raw citrus fruits and their juices are the main source of ascorbic acid for most people. Plants have starch as the storage polysaccharide glucose units held together by glycosidic bonds while animals have the equivalent of glycogen. If you needed an average of 10.

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Vitamin C Determination By Iodine [k546v281gwn8]

vitamin c determination by iodine titration lab report

Label the flask as your vitamin C standard solution. The volume that was required is the starting volume minus the final volume. Ascorbic acid C 6H 8O 6 is a water-soluble vitamin, whose structure is shown in Fig. Label the beaker as your iodine solution. Structure of vitamin C ascorbic acid. After incubation some of this solution shall be taken out with a pipette and inserted into a separate boiling tube containing the same sucrose solution however this solution shall be known as the pre-incubated solution.

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vitamin c determination by iodine titration lab report

Standardization of the Iodine Solution Trial 1 Trial 2 Mass of beaker 48. Packing of whole fruits Volume of fruits needed m 3 of boxes we would have to take to provide a 75mg supply Lime 0. This may require blending or crushing the material and it may also require the addition of known amounts of water to the material. In addition, ascorbic acid has been widely used in the pharmaceutical, chemical, cosmetic and food industry as antioxidant. Many fruits and vegetables contain vitamin C, but cooking destroys the vitamin, so raw citrus fruits and their juices are the main source of ascorbic acid for most people. The instability of ascorbic acid is due to its oxidation to dehydroascorbic acid, which is a reversible reaction, and subsequently to 2,3-diketo-L-gulonic acid.

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