Example of unknown bacteria lab report. Unknown Lab Report 2022-10-09
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An unknown bacteria lab report is a document that describes the process of identifying an unidentified bacterial sample. This type of report is typically used in microbiology labs, where scientists and students are often tasked with identifying unknown bacteria as part of their coursework or research.
To identify an unknown bacterial sample, a number of techniques and tests must be used. These techniques can include physical and chemical characteristics of the bacteria, as well as its growth patterns and ability to survive in different environments.
One example of an unknown bacteria lab report might involve the identification of a bacterial sample that was collected from a patient with a respiratory infection. The sample would be grown in a laboratory setting and observed for its physical characteristics, such as shape, size, and color. The sample might also be tested for its ability to survive in different temperatures, pH levels, and other conditions.
To further identify the bacterial sample, the lab might use biochemical tests to determine the presence or absence of certain enzymes or other chemical compounds. For example, the lab might test the sample for the production of certain enzymes that are characteristic of certain bacterial species, such as lactase or urease.
Once all of the data has been collected and analyzed, the lab can use a combination of physical, chemical, and biochemical characteristics to identify the unknown bacterial sample. The lab report would then include a detailed description of the identification process, as well as any relevant information about the identified bacteria, such as its potential to cause disease and how it might be treated.
In conclusion, unknown bacteria lab reports are an important tool for identifying and understanding the characteristics and behavior of different bacterial species. These reports provide valuable information that can be used to better understand and treat bacterial infections, and they are an essential part of the scientific process.
Unknown Bacteria: Microbiology Lab Report
Bacteria in the oral cavity are known to form biofilms, microbial communities with cell-cell and cell-surface adhesion, which are known to have health implications 1. A new isolation was conducted. Unknown Bacterial Identification Lab Report 7 PURPOSE: The purpose of this lab was to identify two unknown bacteria. For this test, I plated my unknown isolate on a mannitol salt agar MSA plate with S. With the completion of this test, Escherichia coli was confirmed as the unknown Gram negative bacterium. How can we identify bacteria in lab? A Gram positive and Gram negative were isolated off me, while a third known unknown was given to me by the professor, who knew its identity. Bacteria can cause a variety of diseases ranging from something serious to just an inconvenience.
Ultimately, the students in class were tested for their aptitude to determine an unknown organism given to them performing the test that they used throughout the class. Gram staining was the first test that was run to show if the bacteria is gram-positive or gram-negative. The problem was solved. The results for the test can be seen in Figure 3. Following the Gram-negative trail refer to Table 1 , I then performed the capsule stain see Figure 1.
This immediately ruled two of the gram positive bacteria Bacillus cerus and Bacillus subtilis. In order to do this, I first had to isolate DNA from the bacteria by lysing the cells. The gram positive bacteria did not turn yellow meaning acid was not produced, which verifies Staphylococcus epidermidis is the unknown gram positive bacteria. Avila, Maria et al. The bacterium did not produce an alkaline pH, but an acidic one. An additional goal of the project was to learn about the human microbiota and its relevance to health and disease.
Procedures were followed as provided in the general microbiology lab manual by McDonald et, unless otherwise noted. Microorganisms have much longer history than that of mankind. Clinical Infectious Diseases, 32 4 , 605-610. Second, utilizing those techniques allowed for the identification of two unknown bacteria. If a blue color appears, then the organism is oxidase positive. All procedures were followed as stated in the course laboratory manual and also as further elaborated on by Professor Woolam. I was aware that some strains of the bacteria are becoming increasingly resistant to antibiotics, such as methicillin- resistant Staphylococcus aureus MRSA.
Being able to determine the identities of microorganisms is a very important task that can help people in many different settings. . Some of the tests listed above could possibly be different in other text books, which can be expected. There were no problems encountered with finding this conclusion. It is commonly known as being harmful, but this is not always the case. If the article you are reading is about an experiment or study someone other than the writer conducted, then you do not have a primary source.
The slide was then looked at under a microscope. I was able to analyze the sequencing results by using the Basic BLAST function on the NCBI web page. A couple of the unknown bacteria it could have been are Escherichia coli or Staphylococcus aureus. The experiment was done by applying methods in order to identify an unknown bacterium. However, I concluded that I did not have a Micrococcus since those colonies are yellow in color, while my known unknown colony pigment was white.
How do you write an unknown bacteria in a lab report?
A lab report differs from a paper in that it has defined sections. In order to get the Gram negative to grow, another test was performed taking a sample from the original tube using the sterile inoculating loop, and streaking a MacConkey agar placing it in the incubator at 37 degrees Celsius. This test also tests for the presence of H2S which is produced from amino acid breakdown. The plate was placed in the incubator and the bacteria began to grow. In addition, some strains of B. Conversely, a negative result would be no clearing.
In your BIO 150 Lab class you might write the sections of the report individuallyas practice for the final report. It is not recommended to use antibiotics for the treatment of E. May; 143 5 :743-6. A positive result for the particular test our group performed would be reddish-pink and a negative result would be blue. Therefore, I was able to conclude that the known unknown was negative for novobiocin sensitivity, which led me to the end of the flow chart. The purpose of this was to completely isolate the specific bacterium from the mixed colonies on the initial isolation streak plate McDonald et al.
Lab Report Example: Classification of the Unknown Bacterias
This MSA plate was incubated at 37 degrees Celsius for 48 hours. Read all assignments completely, and don't forget to read the rubric grading criteria to know what is expected of you. The reason for this is by the end of all our tests our instructor collected the data online so that we could make a flow chart to differentiate the several organisms at hand by way of several tests. Based on those results, I concluded that my Gram-positive isolate was catalase positive, and was therefore either Staphylococcus or Micrococcus. Retrieved April 25, 2014, from Center For Disease Control and Prevention.
Through the same procedure as the unknown Gram positive see Unknown Gram Positive- Genetic Identification for methods I was Figure 9- API 20E multi- test kit results able to obtain the 16s RNA gene sequence. Several microbiological tests were carried out in order to determine the identity of the unknowns. Wang, Nancy et al. Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction In this paper I will discuss the processes of how I came to find my two unknown bacteria. The gram negative bacteria turned the broth yellow meaning acid was produced, which eliminated the bacteria Proteus vulgaris. For the gram negative bacteria a gelatin test was inoculated to detect if protein was broken down by gelatinase, which it was not eliminating the bacteria Pseudomonas aeruginosa. Unknown 48 was streaked on a mannitol salt agar plate MSA whereas 74 was streaked on an eosin methylene blue plate EMB.