Photosynthesis is the process by which plants, algae, and some bacteria convert light energy into chemical energy in the form of glucose or other sugars. This process is essential for life on Earth, as it provides the energy that drives the vast majority of biological processes. In this lab report, we will describe an experiment designed to investigate the factors that affect photosynthesis in plants and to measure the rate at which photosynthesis occurs.
The experiment was conducted using a controlled environment chamber, which allowed us to manipulate the temperature, light intensity, and carbon dioxide levels in order to study the effects of these factors on photosynthesis. We used spinach leaves as our test plant, as they are easy to obtain and have a high surface area for light absorption.
To measure the rate of photosynthesis, we used a technique called oxygen evolution. This involves measuring the amount of oxygen gas produced by the plant as a result of photosynthesis. We placed the spinach leaves in a solution of water and sodium bicarbonate, which provides the necessary nutrients for photosynthesis to occur. The oxygen produced by the plant was then collected in a gas collection tube, and the volume of gas collected was used to calculate the rate of photosynthesis.
We first tested the effect of temperature on photosynthesis by exposing the spinach leaves to different temperatures ranging from 20°C to 30°C. We found that the rate of photosynthesis increased as the temperature increased, with the highest rate occurring at 30°C. This suggests that temperature is an important factor in photosynthesis, as higher temperatures provide more energy for the chemical reactions involved in photosynthesis to occur.
Next, we tested the effect of light intensity on photosynthesis by exposing the spinach leaves to different light intensities ranging from 50 lux to 500 lux. We found that the rate of photosynthesis increased as the light intensity increased, with the highest rate occurring at 500 lux. This suggests that light intensity is also an important factor in photosynthesis, as more light provides more energy for the chemical reactions involved in photosynthesis to occur.
Finally, we tested the effect of carbon dioxide concentration on photosynthesis by exposing the spinach leaves to different carbon dioxide concentrations ranging from 0% to 10%. We found that the rate of photosynthesis increased as the carbon dioxide concentration increased, with the highest rate occurring at 10%. This suggests that carbon dioxide is an important factor in photosynthesis, as it is used as a reactant in the chemical reactions involved in photosynthesis.
Overall, our results show that temperature, light intensity, and carbon dioxide concentration are all important factors in photosynthesis. By manipulating these factors, we were able to study the effects on the rate of photosynthesis and understand the role that each factor plays in this process. This knowledge can be used to optimize the growth of plants in different environments and to understand the role of photosynthesis in the global carbon cycle.
Biology photosynthesis lab report Free Essays
Chlorophyll is responsible for the absorption of sunlight. This will create a contrast towards the light exposed leaf and will help visualize the role of chlorophyll. First we obtained a leaf from our instructor. Data Of Disks Time m VI. The Effects of Varying Carbon and Light Levels on Photosynthesis Rate Introduction Leaves are other plants depend on light to perform basic cellular functions. The energy is then used to separate water into hydrogen and oxygen and then they combine hydrogen and carbon dioxide to make sugars.
The distance between pigment spots to each wavelength. It is therefore more likely that the data collected was being influenced by the outside light, since there is a significant difference in the number of bubbles from the first run, in which few further light was influencing the production of oxygen. The collected data is noted on paper and will be processed to determine the rate of photosynthesis at every colour. Add drops of Lugol's iodine solution to each leaf until iodine has come into contact with the entire leaf. Leaf disks normally float in solution due to the presence of oxygen.
The leaves being cut for samples were leaves that had been soaking in Chicago River water for two weeks prior to lab. The leaves in the bicarbonate solution did eventually float up, following the water. In this case we have gathered evidence that is based on clear reasoning. At first, the spectrophotometer was left for at least 10 minutes to warm up and after that the wavelength was set at 400 nm. It has two stages or reactions light-dependent and light-dependent reactions.
The uptake of carbon dioxide depending on the amount affects the rate of photosynthesis. Results: Figure 1: The retention value is described, which can be defined as the ratio of the distance moved by the solute and the distance moved by the solvent along the paper. Light is absorbed inside he thylakoid Premium Photosynthesis Plant Oxygen Photosynthesis Lab Report Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. Turgor and the Transport of CO 2 and Water Across the Cuticle Epidermis of Leaves. It is clear that all life forms depend on sunlight for energy to live, to grow, to move, to gather food and to reproduce.
Finally, the light saturation point was found by finding the highest point on the light response curve. The final two thing that were solved for were the photosynthetic efficiency and the light saturation point. When only little light is present causing the plant to produce little starch proves that there is a direct relationship between the rate of photosynthesis and light. Chlorophyll is necessary for photosynthesis, but accessory pigments collect and transfer energy to chlorophyll. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate Premium Photosynthesis Lab Report on Photosynthesis Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis.
An equation was made from this line. Introduction: It is necessary that some information on the experiment on chromatography of plant pigments is given, before the analysis of it. Approximately 20g of leaves without the main veins were weighed. It is a highly complex process that needs to be broken down in many steps to understand how it works. What did you see? The light reactions of photosynthesis.
This will be done by calculating the mean values for all runs of each of the colours by using Excel. It will be shown in the discussion whether the hypothesis made was correct or incorrect. We inserted the straw into a test tube, and blew gently to release carbon dioxide into the tube. Both the oxygen evolution slope and the irradiance level were shown to decrease over time. Apart from the paper chromatography method, another method called spectrophotometry was used in order to measure the absorption of light of each pigment.
Biology Lab Report on the Effects of Photosynthesis Essay Example
. Light independent reactions use carbon dioxide and the products of light reactions ATP and NADPH to form compounds such as glucose Premium Photosynthesis Oxygen Carbon dioxide Photosynthesis Lab Report Maria Ruiz Feb. Photosynthesis occurs in many organisms Premium Photosynthesis Oxygen. Overall, we believed my experiment was a success. With a glass-marking pencil, label one 250-mL beaker light, and label another 250-mL beaker dark. Then we placed the chromatography strip in the test tube, positioning it so the tip of the strip barely touched the solvent.
Next, leaves were de-veined and placed under a lamp as the stem plays no role in photosynthesis, III. Bring the alcohol to boiling, and boil gently until all the chlorophyll in the leaves has dissolved in the alcohol. All the oxygen within the leaf disks will be removed before being placed into either of the solutions. Investigations have shown that green plants involve two photosystems during photosynthesis Arnon, 1971, 2883. Using a hole puncher, we cut 20 holes per test in the spinach leaves to make leaf disks. The materials needed are: at least three leaf specimens collected from a nearby source and a piece of typing paper. The first observations of the phenomenon of photosynthesis were made by Priestley, in 1771.
In Part II, why did you test leaves that were all green as well as leaves that were part green and part white? Now the sunlight ultimately powers the process of photosynthesis. The mean for the dark tube was 9%, while the mean for the unboiled is 15% Table 2. The aim the study that was looked at was to analyze factors that contributed to increased photosynthesis in diffused light Li, et al. Then dip a capillary tube into the plant pigment extract spinach pigment extract provided by the teacher. The start button was clicked to start recording data, and it was not stopped until all data was collected.