Gram staining observation result and conclusion. What is the conclusion for Gram staining? 2022-10-11

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Korean Airlines Flight 801 was a scheduled flight from Seoul, South Korea to Guam that crashed on August 6, 1997, killing 228 of the 254 people on board. The crash of Flight 801 was a tragic and devastating event that had a lasting impact on the families of the victims, as well as on the aviation industry as a whole.

The flight was operated by a Boeing 747-300 aircraft and was piloted by Captain Park Chang-Kyung, a veteran pilot with over 13,000 hours of flying experience. The plane took off from Seoul's Gimpo International Airport at 10:58 p.m. local time and was scheduled to arrive in Guam at 4:01 a.m. the following morning.

However, just minutes after takeoff, the plane encountered heavy rain and strong winds. As the plane flew over the Pacific Ocean, it encountered severe turbulence and the pilots struggled to maintain control. Despite their efforts, the plane crashed into a hillside near Nimitz Hill, just a few miles from the airport in Guam.

The cause of the crash was later determined to be pilot error. The pilots had failed to properly execute the approach to the airport and had not followed the proper procedures for landing in poor weather conditions. In addition, the pilots were found to have inadequate training in the use of the plane's instrument landing system (ILS), which is a critical tool for navigating and landing in poor weather conditions.

The crash of Korean Airlines Flight 801 was a tragic reminder of the importance of proper training and safety procedures in the aviation industry. It also highlighted the need for improved communication and coordination between pilots and air traffic controllers in order to prevent similar tragedies from occurring in the future.

In the wake of the crash, Korean Airlines implemented a number of safety improvements, including increased training for pilots, improved communication and coordination between pilots and air traffic controllers, and the adoption of new technology to help navigate and land planes in poor weather conditions.

The families of the victims of the crash were also left to deal with the aftermath of the tragedy. Many of them filed lawsuits against Korean Airlines, seeking compensation for their losses. In the end, the families of the victims received a settlement from the airline, though it could never fully compensate for the loss of their loved ones.

The crash of Korean Airlines Flight 801 will always be remembered as a tragic and devastating event. It serves as a reminder of the importance of safety and proper training in the aviation industry and the need to continually strive for improvement in order to prevent similar tragedies from occurring in the future.

What is the conclusion of the gram staining technique?

gram staining observation result and conclusion

This differential staining technique separates most bacteria into two groups based on cell wall composition. This staining technique uses two stains; crystal violet as primary stain and safranine as a counterstain. Both Gram-positive and Gram-negative cells will be stained purple by the crystal violet dye. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Introduction Gram staining is the most important differential staining method used in microbiology.

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Conclusion gram staining Free Essays

gram staining observation result and conclusion

This causes them to appear red to pink under a Gram stain. Gram staining is a differential bacterial staining technique used to differentiate bacteria into Gram Positive and Gram Negative types according to their cell wall composition. Gram staining technique is not recommended for spirochetes and mycobacteria. Using this staining technique, bacteria can be differentiated into two groups hence; it is called the differential staining technique. In the case of Gram-negative bacteria, the alcohol, being a lipid solvent, dissolves the outer lipopolysaccharide membrane of the cell wall and also damages the cytoplasmic membrane to which the peptidoglycan attaches. The gram positive cell wall has a thick mesh like cell wall made up of peptidoglycan. The modified procedure provides greater reagent stability and better differentiation of organisms.

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Gram Stain: Introduction, Principle, Procedure, Result and Interpretation

gram staining observation result and conclusion

Further study with more increase in sample size is needed to prove the effectiveness of the modified method. Staining Results and Conclusion I. CONCLUSION: The Gram stain method is a rapid and cost-effective test that is also highly reproducible and readily available in many laboratories. The graphical representation is given in In modified gram stain, we used crystal violet as the primary stain and decolouriser as acetone alcohol mixture with reduction in the staining time. This is because the background staining intensity is highly improved in the modified stain by reduction in the counter staining time to 30 seconds. Clostridium perfringens Boxcar-shaped gram-positive bacilli Gram-variable or Gram-negative bacilli Maybe mistaken for gram-negative bacilli; the boxcar shape is a clue that the organism is gram-positive; other Clostridia and Bacillus spp.

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Lab 5

gram staining observation result and conclusion

Phone: +36 180 38 002, Email: More Locations No part of this content may be reproduced or transmitted in any form or by any means as per the standard guidelines of fair use Best viewed in Mozilla Firefox Google Chrome Above IE 7. J Bacteriol Mycol Open Access. Gram Staining Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. In Gram Staining, it is used as a basic dye in the ionized form of CV+ and Cl-. Keywords: gram stain, modification, staining intensity, diagnosis Introduction The gram stain is one of the most frequently used diagnostic tests.

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Gram Staining: Principle, Procedure, Results • Microbe Online

gram staining observation result and conclusion

It also helps indentify if the unknown organism is a Gram positive or Gram negative. Escherichia coli Procedure: 1. If bacteria are present on a Gram stain slide, a medical laboratory scientist classifies them as gram-negative or gram-positive based on which color they turn under a series of stains. Crystal violet is a dark blue to purple dye. The strength of the binding from the stains depends on the make-up of the cell wall itself. Counter Stain Safranin : It is a red counterstain used in the Gram Staining procedure to stain decolorized Gram-Negative cells. He help to distinguish Klebsiella pneumonia to pneumococci.

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Gram Stain Purpose, Procedure, Results and more

gram staining observation result and conclusion

Overview What is a Gram stain? A note from Cleveland Clinic Gram stains are a very common and often painless way to determine the presence of a bacterial or fungal infection. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM American Society for Microbiology , Washington DC. Positive Gram stain If your test result reveals a positive Gram stain, it means that bacteria were present in your sample. In microbiology and molecular biology, it is used for staining bacteria, histological slide staining, DNA staining, etc. What will be the colors of Gram positive and Gram negative bacteria at the conclusion of the technique? It acts as a lipid solvent and as a protein- dehydrating agent. A Gram stain involves applying a stain to a sample in glass microscope slides and looking at it under a microscope to determine if bacteria are present at all.

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Conclusion In conclusion Gram staining method is widely use to differentiate

gram staining observation result and conclusion

Broadway Edmond Oklahoma 73034. Why is it important to heat fix the bacteria prior to the staining procedure? When fluid samples for example urine or CSF is receive it look to disappear after the staining process, a wax spot, positioned near the smeared part on the Same sideon the glass slide after the staining process to escape from wax artifacts will decrease prevention in finding the sample below the microscope. Methanol fixation is recommended rather than heat fixation. With ethanol treatment, gram-negative cell walls become leaky and allow the large CV-I complexes to be washed from the cell. They have an outer membrane which contains lipids which is separated from the cell wall by periplasmic space.

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Gram Stain: What It Is, Purpose, Procedure & Results

gram staining observation result and conclusion

Most stains can be used on non-living fixed cells, while only some types of stain can be used on living cells. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram staining is not a simple procedure as it used more than one chemical reagent to smear it. The medical laboratory scientist then puts together a report and sends it to your healthcare provider. Thorough research 20% 1. What are the risks of a Gram stain test? One set labeled as original gram and the other set labeled as modified gram. Some strains are obligate anaerobes.

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