Liver and hydrogen peroxide lab report. Hydrogen Peroxide Experiment Lab Report 2022-10-29

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The liver is a vital organ that plays a crucial role in many bodily functions, including the metabolism of nutrients, the detoxification of harmful substances, and the synthesis of important molecules such as bile and hormones. In this lab report, we will be exploring the effects of hydrogen peroxide on liver cells.

Hydrogen peroxide (H2O2) is a strong oxidizing agent that is commonly used as a disinfectant and bleaching agent. It is also produced by the body as a byproduct of various metabolic processes, and it is usually quickly broken down by enzymes such as catalase.

Catalase is an enzyme found in many tissues, including the liver, that catalyzes the breakdown of hydrogen peroxide into water and oxygen gas. This reaction is important for removing excess hydrogen peroxide from the body, as it can be toxic if it accumulates to high levels.

To test the effects of hydrogen peroxide on liver cells, we performed an in vitro experiment using liver tissue from a rat. We isolated liver cells from the tissue and incubated them in a solution containing different concentrations of hydrogen peroxide for various time periods.

We then measured the activity of catalase in the liver cells using a spectrophotometer. This instrument measures the amount of light absorbed by a sample, which allows us to determine the concentration of a particular substance in the sample.

Our results showed that the activity of catalase in the liver cells increased as the concentration of hydrogen peroxide increased. This suggests that the liver cells were able to effectively break down the hydrogen peroxide, indicating that the cells were healthy and functioning normally.

However, we also observed that at very high concentrations of hydrogen peroxide, the activity of catalase began to decrease. This may be due to the toxic effects of hydrogen peroxide on the liver cells, which can cause cell death or damage.

In conclusion, our results demonstrate that the liver has the ability to effectively break down hydrogen peroxide using the enzyme catalase. However, very high concentrations of hydrogen peroxide may be toxic to liver cells and may cause damage or death. These findings have important implications for understanding the effects of hydrogen peroxide on the liver and how it may impact human health.

Liver Enzymes and Hydrogen Peroxide

liver and hydrogen peroxide lab report

The initial Control: The amount of hydrogen peroxide will be kept the same. Enzymes work within an optimal temperature and optimal pH. An effected way is to use tweezers when placing the liver in the test tube. If hydrogen peroxide comes into contact with this, the chemical can transform into water or evaporate as oxygen gas. It helps in preventing the damages done to the cell oxidative and gives a detailed view of atomic and electronic aspects. The Enzyme is usually much larger in size than the substrate it binds to. In addition to the above, catalase acts as the driving force which is an enzyme and leads to the decomposition of hydrogen peroxide into oxygen and water.

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The Effect of Hydrogen Peroxide on Liver

liver and hydrogen peroxide lab report

Enzymes are known to increase the rate at which a chemical reaction occurs. What happens when hydrogen peroxide is added to the liver? When this reaction occurs, oxygen gas bubbles escape and create foam. The liver in a solution of pH 10. Fill the bucket and graduated cylinder with water so that there is no air in the cylinder 3. That is what it takes to understand how the world works, even if the experiment isn't pretty.

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Liver Lab Experiment

liver and hydrogen peroxide lab report

Constants: The size of the test tubes, The size of the pieces of liver, the amount of H2O2 solution added , The type of water, The type of liver, the concentration of H2O2. Make sure you have 8-10 glasses of water daily. The diagram above shows that before osmosis occurs, the two solutions are separated by a partially permeable membrane. Catalase is an enzyme that speeds up the reduction of hydrogen peroxide, or H 2 O 2 which is a natural byproduct of many of the reactions that occur in the human body. Adding in 1 or 2 more set temperatures to be tested such as 20oC and 40oC would also give an overall overlook of the reaction rate of enzymes. Place one piece of liver in the 250 ml flask and then add 3ml of hydrogen peroxide. For any other use, please contact Science Buddies.

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Hydrogen Peroxide Experiment Lab Report

liver and hydrogen peroxide lab report

The rate increases rapidly in the presence of catalysts such as MnO2 and KI. When this reaction occurs, oxygen gas bubbles escape and create foam. This shed light on understanding the effect of catalase on the rate of reaction and the increment and decrement values. They can either launch a reaction or speed it up. All living things have enzymes. They can be very fussy, and sometimes need to be in certain environments or conditions to work, and the ideal conditions are usually reflective of where the enzyme must normally function in the body. One protein that is fun to use for the experiment is Jell-o, made out of gelatin.

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Lab Report

liver and hydrogen peroxide lab report

As the temperature increases toward the optimum point, hydrogen bonds loosen, making it easier for catalase to act on hydrogen peroxide molecules. This includes lack of technical expertise or lack of vigilance while performing the experiments by the scholar which might lead to inaccuracies. Introduction Liver and Hydrogen Peroxide experiment Aim: To see how different concentrations of hydrogen peroxide affects the enzyme activity and rate of reaction. On the large plate, test a drop of the treated liver with hydrogen peroxide. It can break apart to yield hydroxyl radicals that attack important biochemicals like proteins and DNA.

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What happens when you put hydrogen peroxide on liver?

liver and hydrogen peroxide lab report

Does High ALT mean liver damage? Is liver damage painful? Why does potato react with hydrogen peroxide? Heat covered on high for 20 seconds. After that was completed, the KHP was put in a beaker and filled up using 50mL of demonized water and mixed well until the KHP dissolved well in the water. When the AST is higher than ALT, a muscle source of these enzymes should be considered. This was shown by the bubbles in the top of the test tube. Catalase is a key enzyme which uses hydrogen peroxide, a nonradical ROS, as its substrate. Keep the blended liver in the refrigerator. Extracts from this document.

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Hydrogen Peroxide Lab Report

liver and hydrogen peroxide lab report

What part of the body itches with liver problems? This experiment was texting the surface area of the liver. The second experiment performed, experiment 7. What happens when hydrogen peroxide is added to an enzyme? The effects of In a liver sample were observed under iced, boiling, 37 degrees, and room temperature Conditions. In other words, each enzyme has a particular type of reaction that it can activate. Record you observations Approximately 10-15 minutes d. The graph shows that 37oC was the optimum temperature and producing the greatest height of reaction 44mL.

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Liver and Hydrogen Peroxide experiment

liver and hydrogen peroxide lab report

Test the effect of hydrogen peroxide on different materials around your house, what happens? What enzyme from the liver breaks down peroxide? The time the liver reacts with the solution varied with the sample of 3 for each temperature. This disrupts the 3d structure of the protein, causing a permanent changeā€¦ Enzyme Lab Report In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The rate of decomposition is slow at room temperature, but it increases with temperature. Number of Trials: 1 per each test tube because of the limited time frame given Conclusion According to our data, our hypothesis was correct and that the increase of temperature also increases the reaction rate of the H2O2 with the test tube solution. This activity is due to a special protein produced by the potato to protect itself against oxidative stress. Because the reaction initiates as soon as the reactants make contact catalase activity would be higher than the true value in the results. In other words, different tissues are home to different enzymes, and an enzyme should be able to function in the conditions that surround it in the tissue it's supposed to function in.

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Why does liver react with hydrogen peroxide?

liver and hydrogen peroxide lab report

Larger particles decreased the rate of reaction because they require more energy to break down. After that, we placed the test tube filled with catalase in a boiling water bath for five minutes. The remainingā€¦ Enzyme Activity Lab Report In this lab we tested the effect of temperature has on the rate of enzyme activity. While the pure samples of liver were the same surface area, not all pieces were blended from the same section of the Lab: The Effect Of Temperature On Enzyme Activity This may have contaminated the solution, which would result an increase or decrease of catalase activity. A larger amount of catalase lowers the activation energy, therefore speeds up the rate of reaction.

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Liver Enzyme Lab

liver and hydrogen peroxide lab report

Catalase is an enzyme in the liver that breaks down harmful hydrogen peroxide into oxygen and water. Why is hydrogen peroxide harmful to the body? When peroxide bubbles does it mean infection? The potato contains less of the enzyme catalase, therefore requires more activation energy, slowing down the rate of reaction. The labels were necessary because each tube was then placed in its respective temperature zone among tubes from other groups. These drops release oxygen in the earwax, causing it to bubble. Dependent Variable: The reaction rate of the enzyme to the H2O2 solution added. How do you cleanse your liver fast? Then repeat this 9 more times but with weights of 0.

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