Catalase is an enzyme that is found in nearly all living organisms and plays a crucial role in the breakdown of hydrogen peroxide, a toxic byproduct of cellular metabolism. In this lab report, we will explore the factors that can affect the activity of catalase, including temperature, pH, and substrate concentration.
To begin, we prepared a solution of hydrogen peroxide and added a small piece of potato, which contains catalase, to the solution. We then measured the amount of oxygen gas produced over a period of time, as the breakdown of hydrogen peroxide by catalase releases oxygen gas as a byproduct. This served as our control experiment, in which we measured the baseline activity of catalase under standard conditions.
Next, we conducted a series of experiments to test the effect of temperature on catalase activity. To do this, we prepared a series of hydrogen peroxide solutions at different temperatures and added potato pieces to each one. We found that catalase activity increased as the temperature increased, up to a certain point. Above this optimal temperature, the activity of the enzyme began to decrease.
We also tested the effect of pH on catalase activity by preparing hydrogen peroxide solutions at different pH levels and adding potato pieces to each one. We found that catalase activity was highest at a pH of around 7, and decreased as the pH increased or decreased from this optimal level.
Finally, we tested the effect of substrate concentration on catalase activity by preparing solutions of hydrogen peroxide at different concentrations and adding potato pieces to each one. We found that catalase activity increased as the substrate concentration increased, but eventually reached a plateau as the concentration of hydrogen peroxide became too high for the enzyme to effectively break down.
Overall, our results showed that temperature, pH, and substrate concentration can all have an effect on the activity of catalase. Understanding these factors can help us to better understand the role of this important enzyme in cellular metabolism and its potential applications in biotechnology.
Our second experiment demonstrated that as temperature increases the rate of the chemical reaction decreases. Enzymes are produced by cells and they act as catalysts, which means they are organic molecules that speed up chemical reactions. This lab report is worth 100 points towards your final lab grade. Zinc Finger Gene In Sea Lion 806 Words 4 Pages The sample was then incubated at 56°C to lyse the tissue. Effect Of Temperature On Amylase 1167 Words 5 Pages Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. Catalase Enzyme Lab Report Guy Guarino Abstract Enzymes are protein catalyst that speed up the rate of a chemical reaction.
Peroxidase Lab 224 Words 1 Pages The purpose of this study is to investigate the effects of varying the concentration of peroxidase on rate of reaction, as well as, the varying temperature and pH levels. The time needed for the filter paper to float to the top from the bottom of the reaction beaker was measured using a stopwatch and recorded. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to Peroxidase Lab 224 Words 1 Pages The purpose of this study is to investigate the effects of varying the concentration of peroxidase on rate of reaction, as well as, the varying temperature and pH levels. Materials: 3% hydrogen peroxide, a household blender, potatoes 3 medium size potatoes, that are peeled and quartered , tap water, 8 test tubes, 8 transfer pipets, sugar packets, salt, a small saucepan, a refrigerator with a freezer, stove The Effect Of Temperature And Ph Levels On Enzyme Activity Changes in Temperature and pH Levels Affect Enzyme Activity? The amount of sulphuric acid used was also controlled by measuring 100mL with a 100mL measuring cylinder to ensure that the results would be consistent. Introduction Enzymes are protein catalysts, and a catalyst is a substance that speeds up the rate of a chemical reaction without being used up.
Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. During the first step, the egg was put into the vinegar. When a substrate binds to an active site on an enzyme, a reaction occurs 1. Enzymes catalyze most if not all chemical reactions that take place within living cells Morris, 2011. The solution turned a bright yellow color after the hydrobromic acid was added. It is a clear liquid and is used as a disinfectant, antiseptic, and oxidizer.
Effect Of Enzyme Concentration On Enzyme Reaction 1046 Words 5 Pages ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. The active position of an enzyme recognizes, restricts and orients the substrate in a specific direction. Enzymes also known as biological catalase, originate from a living organism in order to act as a catalase to boost Catalase Enzyme Activity Lab Report Abstract The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. How effectively they work depends on the conditions they are in, such as level of temperature, pH level, substrate concentration, and salt concentration. I selected Copper Sulphate as enzyme inhibitor against the concentration of 2% of Amylase solution. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas.
At high temperatures, an enzyme denatures or changes shape, making it difficult or impossible for a substrate to bind, and at low temperatures, the frequency and rate of reaction decreases causing for a halt in product formation Pitzer et. In order to do so we tested for the presence of organic molecules. After one recorded minute, the height of the foam produced was measured and recorded in millimeters. To do Enzyme Lab Report The purpose of the enzyme lab conducted was to observe the chemical composition of cells. Without enzymes, chemical reactions will occur too late to sustain life.
Regarding pH, enzymes are dependent on a proper pH level specific to the reaction and desired outcome 3. The first part of the experiment where the concentration of catalase was altered began by preparing the enzyme concentrations in the 100 ml beakers and labeling them based on the enzyme concentration: 20%, 40%,60%, 80%, and 100%. This experiment used 11 solutions contained in cuvettes. Results Images : Table of Results with Reaction Rate: Glass Amount of Vinegar mL Rate of Reaction 0-5 1 0 3 2 125 0 3 14 2 4 29 1 5 44 0 Analysis: The experiment yielded 5 results and related to the purpose of this lab, to investigate and experiment with the effects of hydrogen peroxide and acetic acid vinegar in regards to denaturing the enzyme, catalase, which is present in potatoes. Catalase is a specific type of enzyme that breaks down Hydrogen Peroxide into water and oxygen.
Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Most enzymes have a preferred temperature and pH range 2. An enzyme, a biological catalyst, works to speed up chemical reactions. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour. Although hydrogen peroxide is produced to help defend against infections, a high level of this poisonous chemical could potentially harm the cells. Peroxidase is the most reliable and accessible enzyme to use as it can be easily prepared and tested Ramesh Kumar 1.
Proteins are next tested. Amylase Lab Report 755 Words 4 Pages Enzymes are biological catalysts, which are essential for carrying metabolic reactions in the human body including the breakdown of food for digestion, absorption and energy production. The second glass contained no substrate, only vinegar to observe what would occur if the catalase was completely submerged into a highly acidic solution. All biological reactions within human cells depend on enzymes Wolfenden 1. To maintain hydrogen peroxide levels the catalase enzyme deconstructs hydrogen peroxide and reconstructs the reactants into oxygen gas and water.
The substrate of catalase is hydrogen peroxide and its main reaction is to decompose it into oxygen and water 1. At a pH of 3 and 11, the enzyme activity greatly decreased, while at pH 7, the enzyme activity was the highest. Other purposes of the lab were; to measure the effects of changes of temperature, pH, enzymes concentration, and substrate concentration on rates of an enzyme. The active site is the location where catalysis occurs. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.
Moreover, the activity levels of enzymes are greatly impacted by temperature and it should be further investigated through scientific applications with The Biological Temperature Of Catalase 750 Words 3 Pages These factors include the pH and the temperature of the solution 1. Introduction The breakdown of Hydrogen Peroxide H2O2 occurs at the same rate in all cells, and the catalase activity is not affected by temperature. These results accurately relate to the hypothesis, in which it was hypothesized that the catalase would become denatured through the addition of acetic acid, which was true. The experiment results show that an increase in the amount of the enzyme, catalase, will lead to a decrease in the time necessary for the substrate, hydrogen peroxide to be broken down. They all lack the enzyme to help determine the absorption of just the enzyme. Abstract: Enzymes, molecules that speed up chemical reactions, are specific to one substrate. The 60% catalase solution was obtained and a disk was dipped, using forceps, into the 60% potato solution for five seconds.