Dissolved oxygen in water lab report. (PDF) Dissolved Oxygen in Water 2022-10-11
Dissolved oxygen in water lab report
Dissolved oxygen (DO) is a measure of the amount of oxygen gas (O2) that is dissolved in water. It is an important parameter to consider when assessing the health of aquatic ecosystems, as many aquatic organisms rely on a certain level of DO to survive. The DO level in water can be influenced by a variety of factors, including temperature, pH, and the presence of algae and other photosynthesizing organisms.
In order to determine the DO level in water, a laboratory procedure called the Winkler method is commonly used. This involves the addition of a chemical reagent to a water sample, which reacts with the dissolved oxygen to form a precipitate. The amount of precipitate formed can then be measured and used to calculate the DO concentration.
To begin the Winkler method, a water sample is collected and brought back to the lab. It is important to handle the sample carefully in order to avoid contamination and to accurately represent the DO level in the natural environment. The sample is then filtered to remove any suspended particles, which could interfere with the chemical reaction.
Next, the chemical reagent is added to the sample. This reagent typically consists of manganese sulfate and an alkaline solution, such as sodium hydroxide. The manganese sulfate reacts with the dissolved oxygen in the water to form manganese oxide, which precipitates out of solution. The alkaline solution helps to dissolve the manganese oxide and make it easier to measure.
Once the chemical reaction is complete, the precipitate is allowed to settle to the bottom of the container. The amount of precipitate can then be measured using a spectrophotometer, which measures the amount of light absorbed by the solution. The DO concentration can be calculated based on the amount of precipitate present.
It is important to calibrate the spectrophotometer using a known standard solution before performing the DO measurement. This ensures that the instrument is properly functioning and that the results are accurate.
The DO level in water is an important indicator of the health of aquatic ecosystems. High DO levels are typically associated with healthy ecosystems, as they indicate the presence of photosynthesizing organisms and good water quality. Low DO levels, on the other hand, can be indicative of pollution or other problems in the water. Monitoring DO levels in aquatic environments can help scientists and resource managers identify potential problems and take steps to address them.
In conclusion, the Winkler method is a reliable and commonly used method for determining the DO level in water. By accurately measuring DO levels, we can better understand the health of aquatic ecosystems and take steps to protect and preserve these important resources.
After pouring the distilled water to the graduated cylinder containing the FeCl3. It also demonstrated the effect of light and nutrients on photosynthesis. Materials Measurement of Dissolved Oxygen This part of the lab required a sample bottle of water from a natural source, a BOD bottle, thermometer, mangonous sulfate, alkaline iodide, thiosulfate, a 2-mL pipette, sulfuric acid, a 20-mL sample cup, a white piece of paper, starch solution, and a nomograph. The cup was placed on a white sheet of paper so that the color changes could be observed. After that, the water sample was fixed using 2ml manganous sulfate and alkaline iodide.
(PDF) Dissolved Oxygen in Water
This absence of sufficient oxygen in the water samples is an indicator of poor water quality, which may require further investigation. John Wiley, Hoboken, NJ Dissolced Oxygen and Water. Heat the solution until all the blue solid has been decomposed to the dark colored copper II oxide. The amount of respiration in all of the bottles exceeded the amount of photosynthesis occurring. According to Fondriest Environmental, Inc , dissolved oxygen refers to the level of free, non-compound oxygen present in water or other liquids. Before the titration of water sample, all the solution and reagent was prepared, the groups collected water sample using BOD bottles. Methods Measurement of Dissolved Oxygen The sample bottle was filled completely so that there were no air bubbles in the bottle.
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The bottles were submerged for about 30seconds avoiding introduction of air bubbles and turbulence. The raising of the air or water temperature by artificial means is called thermal pollution. A concentration of dissolved oxygen less than 4ppm is stressful to most forms of aquatic life. Experiment 7 Dissolved Oxygen DO by the Winkler Method are waterfalls or rapids, and decreasing in the slow-movin are waterfalls or rapids, and decreasing in the slow-movin Introduction Dissolved oxygen in water is one of the foremost imperative parameters in identifying its quality, since it indirectly shows whether there's a few kind of contamination. Pipet 2 mL 0. When there is something added a certain color appeared such as the brown precipitate when Manganese sulfate was added, Alkaline- Iodide reagent for white colorization, golden color in Sulfuric acid, and the blue pigments when the starch was added for indication.
AP Sample Lab 12 Dissolved Oxygen
The concentration of the acid was 0. Measurement of Primary Productivity A second sample bottle was filled from a natural source making sure there were no air bubbles. This left no quantifiable data to use in graphs and tables. RESULTS AND DISCUSSION Table 1. Standards one through six contained the following mL of original stock solution: 1.
Dissolved Oxygen Laboratory Report, Sample of Essays
The physical color change was demonstrated through the formation of the precipitate. Temperature also has an affect, as temperature rises, solubility decreases. The amount of MnSO4 is not critical for the fixation of the dissolved oxygen. If any spattering occurs while heating, wash the solid back down into the solution using a wash bottle. It has an objective to determine the concentration of dissolved oxygen in water and to analyzes the involved chemicals used in understanding the changes in the experiment using the Winkler method.
Dissolve Oxygen Lab Report
There are several factors that effect the dissolved oxygen levels in aquatic environments. The solution turned clear when acids and anhydrides was added, which indicated the precipitate were dissolved. In the preparation of 2. Low levels of dissolved oxygen in water can cause the deaths of thousands of marine animals. Eight drops of mangonous sulfate were added to the bottle. No solution could filter through until it was lifted. Excess pollution or dumping of wastes into the water sample is a suspected cause of the poor water quality.
Dissolved Oxygen Test
Figure 2: The This shows that at the References Heguenin, J. There are other reasons that kills fish, such as the discharge of electrical power plants. Wind allows oxygen to be mixed into the water at the surface. In test for Phosphate, the standard solution was the Phosphate solution and the reagents used were concentrated H2SO4, concentrated HNO3, 2. The independent variable of this experiment is the oxygen concentration in which the Daphnia will be placed in and the dependent variable of the experiment is the hemoglobin produced in the Daphnia. Equilibrium Lab Report 1249 Words 5 Pages Prepare the standard solution of FeSCN2+ pipetting 18 mL of 0.
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Hypothesis The temperature and amount of light an aquatic environment receives greatly affects the dissolved oxygen levels, along with the amount of primary aquatic productivity. One drop of the titrant was added at a time until the color changed to a pale yellow color. Laboratory manual for principles of general chemistry 8th ed. Get 25 mL of 3M KOH solution, add 5 mL KOH solution into the beaker and stir the beaker. As temperature rises, dissolved oxygen levels decrease. In order to obtain only magnesium oxide, distilled water was added so that magnesium nitride will react and convert to magnesium hydroxide.