Unknown bacteria lab report. Unknown Bacteria Lab Report 2022-10-06
Unknown bacteria lab report
An unknown bacteria lab report is a document that presents the results of an experiment in which the identity of a bacterial sample is determined. The purpose of this experiment is to familiarize students with the techniques used to identify and classify bacteria, as well as to understand the importance of accurately identifying bacteria in a variety of settings, including medical, industrial, and environmental.
The first step in an unknown bacteria lab report is to obtain a bacterial sample. This can be done through a variety of methods, including culturing bacteria from a patient, collecting samples from the environment, or obtaining a pure culture from a laboratory supply company. The sample should be handled and transported in a manner that prevents contamination, as this can affect the accuracy of the results.
Once the sample has been obtained, it is important to prepare it for testing. This may involve diluting the sample, plating it onto agar media, or performing other steps to ensure that the bacteria are viable and able to grow.
Next, a series of tests are conducted to determine the identity of the unknown bacterial sample. These tests may include biochemical tests, such as the metabolism of glucose or the production of certain enzymes, as well as physical tests, such as the shape and size of the bacteria or the presence of certain structures. The results of these tests are recorded and used to narrow down the possible identities of the bacterial sample.
Finally, the results of the tests are analyzed and compared to known bacterial species in order to determine the identity of the unknown sample. This process may involve consulting reference materials, such as bacterial identification guides or online databases, and may require further testing to confirm the identification.
Once the identity of the unknown bacterial sample has been determined, the lab report should summarize the methods and results of the experiment, as well as provide a discussion of the implications of the findings. This may include a discussion of the characteristics and behavior of the identified bacteria, as well as its potential impact on human health or the environment.
In conclusion, an unknown bacteria lab report is a document that presents the results of an experiment to identify and classify a bacterial sample. This experiment is important for understanding the techniques used to identify and classify bacteria, as well as the importance of accurately identifying bacteria in various settings. By carefully following proper protocols and analyzing the results of various tests, it is possible to accurately determine the identity of an unknown bacterial sample.
Unknown Bacteria: Microbiology Lab Report
The laboratory manual, by McDonald, includes all the procedures used for these methods 1. The smear was then gram stained in these four steps After each step, the smear should be gently washed with tap water : a. In order to attain correct identification of my two unknown bacterium I had to perform a series of experiments on them, known as biochemical tests. The first step was the preparation of the medium, the bottom of the BHIA dish was labeled with the bacterium number, initials, and section; then divided into four quadrants. Methods and Materials The initial test that was performed to the unknown bacteria was a Gram stain. However, it was apparent that I had successfully isolated two different bacterial species by examining my MAC and CNA plates. This bacteria can catalyze in many environments which makes it very common and found almost everywhere such as soil, water, humans, plants, sewage and hospitals.
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Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. Gives all cells a purple-black colour b. The correct plates were chosen based on the shape and color of the bacteria under the microscope. Preparing for class - Day 1 Read How to Write a Lab Report in Microbiology WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. They could develop HUS hemolytic uremic syndrome , a type of kidney failure, which can be potentially fatal 2.
How to Write a Microbiology Unknown Lab Report
After isolating one of the bacterium a Gram stain was performed. After removal with bacteria on the loop, the quadrant streak method was used. Then a urea test was performed to check for the production of urease. A lab report differs from a paper in that it has defined sections. Here is an excellent example of how to write an unknown lab report in Microbiology class. A bacteria smear was prepared and first stained with crystal violet for 30 seconds.
Example of a Microbiology Unknown Lab Report
Materials and Procedures The unknowns that were tested were numbers 48 and 74. ABSTRACT The objective of these series of experiments was to identify an unknown bacterium. This gave a positive result showing a hot pink broth, meaning the bacteria did produce urease. It was first discovered in 1885 by pediatrician and bacteriologist, Theodor Escherich 2. This left Bacillus subtilis.
How do you write an unknown bacteria in a lab report?
A few tests that microbiologists will perform are isolation, staining reactions, biochemical reactions, indole tests, Catalase test, Coagulase test, Oxidase test and many others. Unknown 48 was streaked on a mannitol salt agar plate MSA whereas 74 was streaked on an eosin methylene blue plate EMB. Gamma hemolysis, no degradation, will not show a halo. Lab Manual for General Microbiology. In the health field it is important for physicians to understand this so the patient can be accurately treated. Selecting the correct antibiotic for this particular bacteria is especially important.
Isolation of 2 Bacteria
However, like stated earlier, a problem was faced after the isolation was incubated. Ciobanu 2 Identification of Unknown Bacteria Lab Report Objective: The main objective of this lab is to determine the genus and species of an unknown bacteria. Next was the acetone alcohol to decolorize and after rinsed safranin was added as a counterstain. The gram stain showed clear purple gram positive cocci. After observation, a sample was taken from the isolated colony on the streak plate and another streak plate was done with that, trying to further isolate the colonies. Following the nitrate test was the Urea Test to determine if the bacteria produced urease. Clinical Infectious Diseases, 32 4 , 605-610.
Lab Report Example: Classification of the Unknown Bacterias
Results The first test performed on the gram negative bacteria, was a Casein Test. Aerobic bacteria generally produce one or the other. The proper procedure for all of these tests can be found in the laboratory manual. After the Gram stain was completed, the bacteria were streaked on an Eosin -Methylene Blue Agar plate and an Enterotube II was inoculated. Returning to the original unknown stock 123, a quadrant streak plate was done with a sterilized inoculating look on a mannitol salt agar, which inhibits the growth of gram negative bacteria. The only gram negative bacteria that shows a positive result on the Casein Test is Pseudomonas aeruginosa, therefore correctly confirming the unknown gram negative bacteria.
Unknown Bacteria Lab Report
Identifying bacteria can also be used in many other areas from better crop production through microbial pesticides to biological warfare. Even on an MSA agar, it took a few tries and several isolations to get the gram positive to successfully grow. Second, people should know that pasteurized drinks are safer than unpasteurized. The colonies were rough granular , translucent, and white brownish color with black granules. It does not allow gram positive bacteria to grow and indicates lactose fermentation by the color of the colony. The Gram stain showed it was Gram positive rods. The two bacteria that give a positive result for this test are Staphylococcus aureus and Staphylococcus epidermidis.